To determine whether the 3'-UTR of EZH2 mRNA is a functional target of miR-98, miR-101 and miR-214 in ESCC cells, we measured the luciferase activity in cells cotransfected with these miRNAs (or control miRNA) and Luc-EZH2 plasmid (or Luc-EZH2-mut plasmid) in ESCC cell line (Eca109). This evidence concerns the gene EZH2 and esophageal squamous cell carcinoma.