To assess if the expression of ARID3B splice forms is regulated by growth factor signaling (both EGF and platelet derived growth factor (PDGF)), we grew DOV13, OVCA 429 and OVCA 433 ovarian cancer cells under serum starved (SS) or 10%/full serum (FS) conditions for 24 h and treated them with or without 20 nM EGF or 10 nM PDGF for an additional 24 h and analyzed the mRNA expression of ARID3B splice forms by QRT-PCR using 18 S rRNA as a control (Fig. 3A, 3B, 3C and 3D). This evidence concerns the gene EGF and ovarian cancer.