As shown in Figure 3A, two-way ANOVA of the EPM data revealed a significant interaction between CD4+CD25+ Treg cell depletion and CIS (F1, 56 = 5.26, p = 0.0256) and a significant effect of CIS (F1, 56 = 17.43, p = 0.0001), but there was no significant effect of CD4+CD25+ Treg cell depletion (F1, 56 = 17.43, p = 0.2173) on time spent in the open arms. The gene discussed is CD4; the disease is in situ carcinoma.