CD4 and Anxiety: Moreover, the frequency of entries into both arms was unaffected by the interaction between CD4+CD25+ Treg cell depletion and CIS (F1, 56 = 1.53, p = 0.2243), CIS (F1, 56 = 0.127, p = 0.7231) and CD4+CD25+ Treg cell depletion (F1, 56 = 0.301, p = 0.5868), indicating that there were no significant differences in the locomotion independent anxiety levels of the mice (Fig. 3C).