Two-way ANOVA analysis revealed a significant interaction between CD4+CD25+ Treg cell depletion and CIS (F1, 33 = 15.86, p = 0.0004), a significant effect of the CD4+CD25+ Treg cell depletion (F1, 33 = 29.23, p<0.0001) and effect of the CIS (F1, 33 = 13.37, p<0.0001). This evidence concerns the gene CD4 and in situ carcinoma.