As shown in Figure 5, two-way ANOVA analysis of the TST data showed that the durations of immobility did not differ significantly among groups (CD4+CD25+ Treg cells depletion×CIS interaction effect, F1, 56 = 2.17, p = 0.1462; CIS main effect, F1, 56 = 0.522, p = 0.4729; CD4+CD25+ Treg cells depletion main effect, F1, 56 = 0.062, p = 0.8032). This evidence concerns the gene CD4 and in situ carcinoma.