As shown in Figure 8A, a significant effect of CD4+CD25+ Treg cell depletion on the serum concentrations of IL-4 was observed in two-way ANOVA analysis, which differed significantly among groups (F1, 39 = 26.51, p = 0.001), but there were no significant interaction between CD4+CD25+ Treg cell depletion and CIS (F1, 39 = 1.97, p = 0.1689) and a significant main effect of CIS (F1, 39 = 2.43, p = 2.978) was not observed. Here, CD4 is linked to in situ carcinoma.