Concomitant with the data for the open arms, no significant interaction between CD4+CD25+ Treg cell depletion and CIS (F1, 56 = 3.35, p = 0.0065) was observed in the two-way ANOVA, but the CIS led to significantly elevated time spent in the closed arms of EPM (F1, 56 = 6.45, p = 0.0139) without the influence of CD4+CD25+ Treg cell depletion (F1, 56 = 0.097, p = 0.757) (Fig. 3B). Here, CD4 is linked to in situ carcinoma.