TNF and in situ carcinoma: As shown in Figure 6A and 6B, a significant effect of CD4+CD25+ Treg cell depletion on serum concentrations of the monocytic pro-inflammatory cytokines, IL-6 (F1, 39 = 6.86, p = 0.0125) and TNF-α (F1, 39 = 8.97, p = 0.0048) were observed in the two-way ANOVA, but there were no significant interaction between CD4+CD25+ Treg cell depletion and CIS and the effect of CIS on the concentrations of IL-6 (F1, 39 = 1.40, p = 0.2433 and F1, 39 = 3.01, p = 0.0908) and TNF-α (F1, 39 = 2.10, p = 0.1555 and F1, 39 = 2.09, p = 0.0.1471).