The cells were infected at a M.O.I. of 10 to increase the infection rate (Fig. 1a and b) Immunofluorescence analyses (IFA) were performed DV-infected K562 cells to assess the migration of HMGB1 from the nucleus to the cytoplasm of DV-infected cells and representative images are shown in Fig. 1a. Here, HMGB1 is linked to infection.