SPI1 and acute myeloid leukemia: Using the pu.1 promoter to drive transgene expression in myeloid cells, Tg(pu.1:MOZ-TIF2-eGFP) fish were the first to demonstrate overt AML in zebrafish at 14 to 26 months of life, showing an accumulation of immature myelomonocytes in the kidney marrow and a reduction in haematopoietic cells within the spleen [52].