TLR3/7/8 activation during pregnancy had no effect on body weight (Figure 5B), however poly I:C, R-837, and CLO97 all caused splenomegaly, supporting a state of excessive inflammation and immune system activation during pregnancy (spleen weight/body weight-P: 3.0±0.1 mg/g, P+TLR3: 5.7±0.3 mg/g, P+TLR7: 4.6±0.3 mg/g, P+TLR7/8: 5.1±0.3 mg/g; all p<0.05 vs. P; Figure 5C). The gene discussed is TLR7; the disease is Splenomegaly.