NLRC4 and infection: To identify the NLR involved in IL-1β secretion, we infected bone marrow-derived wild type (wt) and knockout macrophages in NLRC4, NLRP3, ASC and Caspase-1 (all from C57BL/6 mice background) at MOI of 50 and quantified the IL-1β produced 24 h post-infection.