To further study the interaction between MMP-2 and αvβ3 integrin, we cultured the human A375 melanoma cells using an agarose drop model with the following steps: 2 μl drops of cells (5×106 cells/ml) in the agarose were seeded onto fibronectin-coated coverslips in a 24-well plate. This evidence concerns the gene FN1 and melanoma.