The immunoblot experiment with the recombinant arginine kinase (Fig. 1, lower panels) showed that 21 of all the patients with IgE to moth extracts (25%) had specific IgE to Plo i 1, and again the groups with mite, mite and dander, and seafood allergies had the highest prevalence and displayed the strongest signals, no Plo i 1 positive reactions were detected in any mite negative patient. This evidence concerns the gene IGHE and allergic disease.