After nine days of treatment with 100 μM acetazolamide in OM, the expression levels of ALP (P = 8.71*10-5), BSP (P = 0.001), OCN (P = 0.007) and Runx2 (P = 5.74*10-5) were significantly decreased in Saos-2 cells compared to their levels in OM alone, except for OSX (P = 0.026). This evidence concerns the gene RUNX2 and ocular melanoma.