The mRNA level of Runx2 was 8.05-fold (P = 3.57*10-7) higher in the stimulated cells than in cells without stimulation, ALP increased 2.91-fold (P = 0.000929), BSP increased 27.28-fold (P = 5.63*10-5), OCN increased 2.48-fold (P = 0.04) and OSX increased 1.8-fold (P = 0.002), when Saos-2 cells were incubated in OM for nine days. The gene discussed is IBSP; the disease is ocular melanoma.