These two transcription factor expressions can be observed co-localized in the same areas of the cell nuclei in some malignant tumors with around 36.4% of the cases showing both Sox2 and Oct4 positive staining and around 88.6% of the cases showing either Sox2 or Oct4 positive staining, indicating that the joint detection of the two factors is helpful. This evidence concerns the gene POU5F1 and cancer.