Third, each of Sox2 and Oct4 alone had some limitation of sensitivity as a biomarker of NSCLC, however, when combined, the sensitivity was significantly improved to 88.6%, which suggests that Sox2 and Oct4 can be regarded as coalition predictive factors for patients with lung adenocarcinoma or SCC, and the significance of co-detection of the two factors was much more sensitive than detection of one factor alone. This evidence concerns the gene SOX2 and lung adenocarcinoma.