SOD1 and amyotrophic lateral sclerosis: To determine whether wild-type SOD1 is indeed oxidized in sALS cases, Guareschi and coworkers have first immunoprecipitated SOD1 from lymphoblasts derived from ALS patients and non-neurologic controls and then analyzed the presence of oxidized carbonyl groups in those immunoprecipitated SOD1 by derivatization with 2,4-dinitorophenylhydrazine (DNPH) [59].