To confirm IL-10 induction in GBS-infected macrophages was linked to inhibition of IL-12 expression, we infected macrophages from IL-10 knockout mice (Il10−/−) with wt GBS; Il10−/− macrophages showed a profound increase in IL-12p40 expression after infection with wt GBS, almost 200 fold within 4 h (Fig.S3A); furthermore, addition of an IL-10 receptor-blocking antibody reversed the inhibition of IL-12 expression by βh/ce in macrophages stimulated with heat-killed 10/84ΔcylE bacteria (Fig.S3B). The gene discussed is IL10; the disease is infection.