TNFRSF11A and breast cancer: While wt TNFRSF11A expression was detected in all breast cancer cell lines tested, the TNFRSF11A_Δ7,8,9 variant was observed only in MCF10A, T47D, MCF-7 and MDA-MB-468 cell lines when conventional PCR and gel electrophoresis were employed (Figure 3A).