To study the behavior of Wnt3a over-expression in an ischemic environment, we used the endothelin-1 ischemia model Endothelin-1 is a potent vasoconstrictor that has been used to induce ischemic injury resembling a thrombo-embolic stroke event [11], [12] when injected directly into rodent brain. This evidence concerns the gene EDN1 and ischemia.