To further validate our data and to rule out possible off-target effects of the siRNA targeting NIPP1 we also examined the electrotactic response of the highly metastatic human prostate cancer cell line, PC-3-M, depleted in NIPP1 levels via expression of a shRNA targeting NIPP1 after IPTG treatment. The gene discussed is PPP1R8; the disease is Familial prostate cancer.