We first confirmed that URGCP/URG4 regulated HCC cell proliferation and the cell cycle, as demonstrated by colony formation assay (see Materials and Methods S1, Figure S2B and Figure 3A), MTT assay (see Materials and Methods S1, Figure S2C and Figure 3B), soft agar (see Materials and Methods S1, Figure S2D and Figure 3C), BrdU staining (see materials and methods S1, Figure S2E and Figure S4A) and cell cycle analysis (see materials and methods S1, Figure S3A, Figure S4B and S4C). Here, URGCP is linked to hepatocellular carcinoma.