It is also of note that the increase in RANKL expression induced by ISO was more pronounced in the MC3T3 osteoblastic cell line when compared to BMSCs, suggesting that among the adherent stromal cells constituting the bone marrow, osteoblasts represent the main target for the effect of sympathetic nerves on RANKL expression and breast cancer cell bone colonization. This evidence concerns the gene TNFSF11 and breast cancer.