Second, endogenous sympathetic activation by CIS, like ISO treatment, significantly increased Rankl expression in bone 2 h post-stimulation in mice that have been subjected to CIS for 2 wk, indicating that the chosen CIS regimen does not lead to desensitization, at least within the early critical period of bone colonization focused on in this study (Figure 4C). Here, TNFSF11 is linked to in situ carcinoma.