We used a Cox proportional hazards model, with death from breast cancer (median BCSS, 25.1 [range, 0.5-53.2 months]) and time to recurrence (median DFS, 21.5 months [range, 0.5-53.2 months]) as the endpoints and tumor grade, LN status, radiotherapy and hormone treatment, ER/PR, HER2, EGFR, TN, VEGF-R1, VEGF-R2, tumor stromal VEGF-A, and tumor stromal VEGF-R1 as the predictive variables. The gene discussed is ESR1; the disease is breast carcinoma.