TGFB1 and tetanus: Indirect pathwayResponder: recipient PBMCStimulator: Do PBMC/spleen cell sonicates, HLA single antigen luminex beads and Tetanus or Diptheria toxinReadout: Trans-vivo DTHAssay for regulation:in vivo linked suppression assay of TTOX/DT antigen ± Do antigen, ±neutralising TGFβ/IL-10 antibodies