ACSL4 and neoplasm: To further assess the role of ACSL4 in the tumorigenic capacity of MCF-7 cells, nude mice were inoculated s.c. with MCF-7 Tet-Off/ACSL4 cells as described in Figure 1 and tumor xenograft development was measured in animals that received doxycycline (2 mg/ml) or vehicle in drinking water.