To gain further insights into the immunopathological mechanisms underlying acute C. jejuni induced enterocolitis in vivo we crossed IL-10−/− mice with animals deficient in TLR-2 or -4 recognizing bacterial lipoproteins and lipooligosaccharide, respectively (all in B10 background, see Methods), and generated gnotobiotic double-deficient mice by quintuple antibiotic treatment for 4–5 months starting right after weaning. Here, TLR2 is linked to enterocolitis.