In this study, we used a proteomic approach together with two-dimensional Southwestern blotting (SWB) and confocal analyses to characterize the bond between NM proteins and MARs in three human PCa cell lines representing models of different stages of PCa progression: the well-differentiated androgen-responsive LNCaP cell line, the intermediate-differentiate 22Rv1 cells expressing androgen receptor (AR) but androgen-independent and finally the poorly differentiated and strongly aggressive PC3 which does not express AR. The gene discussed is AR; the disease is posterior cortical atrophy.