This was not due to ineffective mTORC2 inhibition, but instead rebound AKT phosphorylation: while 100 nM AZD8055 inhibited AKT phosphorylation after 2 hours in all three tumor genotypes, by 24 hours AKT phosphorylation rebounded only in BRAF and GNAQ cells (Figure S2C). This evidence concerns the gene BRAF and neoplasm.