To induce AA amyloidosis, mice, C57bl/6 (Ctr) and heparanase-knockout (Hpa-KO), at the age of 12 weeks were injected with 100 μg of amyloid enhancing factor (AEF) into the tail vein, followed by subcutaneous injection of 0.2 ml of 1% silver nitrate to stimulate inflammation. This evidence concerns the gene HPSE and AA amyloidosis.