As shown in Figures 3G and 3H, a number of genes involved in fatty acid synthesis (ATP citrate lyase, Acly), glyceroneogenesis (phosphoenolpyruvate carboxykinase, Pck1) and mitochondrial biogenesis and function (PPARγ coactivator 1 α (Ppargc1a; PGC1α), endothelial nitric oxide synthase 3 (Nos3), Uncoupling protein 2 (Ucp2)) were down-regulated in Het GIP Tg mice fed the HF diet. The gene discussed is PCK1; the disease is hydrops fetalis.