IGROV1-Luc tumour cells in vitro secreted TNF-α, TNFR1-α, TGF-α, AREG, HB-EGF and IL-6Rα, all known substrates for the shedding activity of ADAM17, into the culture medium even when unstimulated (Figure 1A). The gene discussed is EGF; the disease is neoplasm.