AREG and neoplasm: Human TNF-α, TNFR1-α, TGF-α, amphiregulin (AREG), and HB-EGF (ADAM17 substrates) were detected in the circulation of IGROV1-Luc tumour-bearing mice, with high concentrations in the ascites fluid and lower concentrations in the plasma (as shown in the vehicle control groups of the efficacy study, shown later).