It is also possible that the protocol used to generate NK cells, ex vivo culture in the presence of IL-2, may have masked the effect of PKCθ during the cellular cytotoxicity assays, since the tumor cells used in that study are especially sensitive to NK cells (Van den Broek et al., 1995; Screpanti et al., 2001; Pardo et al., 2002). The gene discussed is PRRT2; the disease is neoplasm.