Firstly, using a high affinitymAb specific for MART-1, 2A9 that detects MART-1 by IHC, immunofluorescenceand Western Blot in melanoma cells, the capacity of HLA-A2+tumor cells to directly stimulate MART-1 specific CD8 T cells could be relatedto the expression level of MART-1. Here, MLANA is linked to melanoma.