MLANA and melanoma: Thus, in thepresent work, we analyzed whether phenotypically well-defined [32] clinical grade human macrophagesprepared ex vivo can cross-present naturally occurring tumor-associated antigen(MART-1) after phagocytosis of apoptotic/necrotic melanoma cells and how theycompare with clinical grade DC [33].Melanoma cell death through gamma-irradiation occurs by induction of apoptosisand secondary necrosis, since after 70 Gy irradiation dying cells consistof a mixture of A+/PI− and A+/PI+ cells, as we have shownhere for gamma-MEL-XY3 cells (Fig. S1).