ACSS2 and posterior cortical atrophy: Although we were unable to detect acetylation of AceCS1 following Nampt inhibition in PCa cells (data not shown), inhibition of sirtuin activity with Sirtinol reduced 14C-acetate incorporation into fatty acids by 57%, 74%, and 92% in PC-3, DU145, and LNCaP cells, respectively (Figure 6B).