Conversely, determining the ability of compounds to modulate the growth of Mtb made hyper-susceptible to DHFR inhibition permits us to identify weaker molecules, thereby gaining valuable information concerning structure-activity relationship to assist the design of effective drug leads against Mtb. With the growing demand for better anti-tuberculosis agents, it is incumbent on us to pursue diverse avenues of drug discovery. The gene discussed is DHFR; the disease is tuberculosis.