To investigate the biological relevance of the data obtained using TLR7 and TLR8 ligands, we infected PBMCs of the same subjects with influenza virus (PR8), a RNA virus known to induce IFN-α production through interaction with TLR7/8 [23], and measured the production of IFN-α 24 hours post infection (p.i). Here, TLR8 is linked to infection.