Several interesting pathways and functions were enriched significantly in the DM2 group compared to CTR, such as “Mitochondrial Dysfunctions”, “TGF-β Signaling”, “Insulin Receptor Signaling”, “Calcium Signaling” for the pathways and “Skeletal and Muscular System Development”, “Cell Cycle”, “Cell Death”, “Skeletal and Muscular Disorders” for the enriched functions (Table S2 and S3). The gene discussed is TGFB1; the disease is muscle tissue disorder.