Conversely, hN1 mAb-treated human T-ALL LIC engrafted mice showed a significant reduction in both CD45+ cell burden (Figure 4D; **, P = 0.01, unequal variance two tailed Student’s t-test) and the CD45+CD34+ leukemic cell population (Figure 4D; *, P = 0.05) in the 2° transplant recipients, indicating that NOTCH1 inhibition abrogates T-ALL LIC self-renewal. Here, NOTCH1 is linked to acute lymphoblastic leukemia.