CD34 and acute lymphoblastic leukemia: To determine if lentiviral luciferase-transduced CD34+ and CD34− cells from NOTCH1Mutated and NOTCH1WT pediatric T-ALL samples differed in their capacity to propagate disease, quantitative non-invasive bioluminescent imaging was performed within 10 weeks of intrahepatic transplantation of neonatal RAG2−/−γc−/− mice (Figures 1A and B).