IRS2 and hydrops fetalis: At week 2 and week 4, respectively, the HF–HS diet decreased the mRNA expression of insulin receptor substrate 2 (IRS2) by 35% (not significant) and 68% (P < 0.01), that of protein kinase B beta (Akt2) by 58% (P < 0.05) and 71% (P < 0.01), and that of 5' adenosine monophosphate-activated protein kinase (AMPK) by 60% (P < 0.05) and 42% (P < 0.01).