Additionally, quantitative real-time PCR analyses revealed that there was a 2.4-fold increase in TRPV4 mRNA 1 hour after H/I when compared to the mRNA content of the hippocampal CA1 region in sham-operated rats (Fig. 2). As expected for an increase in astroglial reactivity, GFAP was gradually increased 3D and 7D after ischemia (Fig. 2and Fig. S1). Here, GFAP is linked to ischemia.