TRPV4 and ischemia: Additionally, quantitative real-time PCR analyses revealed that there was a 2.4-fold increase in TRPV4 mRNA 1 hour after H/I when compared to the mRNA content of the hippocampal CA1 region in sham-operated rats (Fig. 2). As expected for an increase in astroglial reactivity, GFAP was gradually increased 3D and 7D after ischemia (Fig. 2and Fig. S1).