In the present study we demonstrated that lentiviral-based silencing of EGFR efficiently attenuated its pro-mitogenic actions in 3 of 4 pancreatic cancer cell lines, and that lentiviral-based sequestration of TGF-β also attenuated proliferation in 3-D culture in the same three cell lines. Here, TGFB1 is linked to familial pancreatic carcinoma.