The results suggested that the effects of AKT and (or) ERK1/2 on miR-21-regulating EMT and CSC phenotype, in accordance with that AKT and (or) ERK1/2 pathways are required for promote EMT and (or) CSC phenotype in breast cancer MCF-7 cells [32], breast epithelial cells [33], [34], colon cancer cells [35], cervical cancer cells [32], and ovarian carcinomas cells [36]. Here, AKT1 is linked to malignant colon neoplasm.