AKT1 and chondrosarcoma: Everolimus alone did not led to an increase in Akt phosphorylation in the chondrosarcoma model as seen by western blotting and immunofluorescent stainings (Figure 4); in contrast an increase in Akt phosphorylation could be seen by western blot in the doxorubicin treated group (77.4% of Akt was phosphorylated in these tumors) in comparison to the control one where 68% of Akt was in its activated form in the control group.