We also observed upregulated expression of several reticulocyte specific genes such as ankyrin1, erythroid associated factor, hemoglobins, nuclear associated factor, glycophorin, transferrin receptor, and selenium binding protein, as expected with prominent reticulocytosis in SCD, thereby validating the performance of the two technologies in identifying biological alterations in the sickle cell disease model. Here, AHSP is linked to sickle cell disease.