In the hippocampus, no changes in total Akt were observed 54 h after the onset of ischemia (Figure 3D), although a significant decrease in pAkt Ser-473 was detected (72 ± 5.1% reduction vs. SV group, P = 0.005; Figure 3E) and to a lesser extent in pAkt Thr-308 (32 ± 8.5% reduction vs. SV group, P = 0.007; Figure 3F). This evidence concerns the gene AKT1 and ischemia.