In the present study, we evaluated the impact of post-ischemic estradiol treatment in rats on the late stages of cerebral ischemia (54 h post-pMCAO), analyzing the activation of the PI3K/Akt/GSK3/β-catenin pathway and of SAPK-JNK in two brain areas affected by pMCAO: the cerebral cortex and the hippocampus. The gene discussed is MAPK8; the disease is brain ischemia.