Post-ischemic treatment with three doses of estradiol, beginning 6 h after the onset of pMCAO, partially recovered the activity of the PI3K/Akt/GSK3/β-catenin pathway, although this effect was more pronounced in the cerebral cortex (the region most affected by ischemia in this model) than in the hippocampus (secondary death cell area). This evidence concerns the gene AKT1 and ischemia.