Given that isoflurane may induce a greater increase in Tau-PS262 levels in AD Tg mice brain tissues than in WT mice brain tissues, next we assessed the effects of isoflurane on Tau-PS262 levels in primary neurons from WT and AD Tg mice and further asked whether elevated Aβ level may potentiate the isoflurane-induced increase in phosphorylated tau levels. Here, MAPT is linked to Alzheimer disease.