We did not measure the effects of isoflurane on total tau levels in brain tissues of AD Tg mice because the isoflurane anesthesia did not increase total tau levels in brain tissues of WT mice and we only wanted to compare the effects of isoflurane on brain phosphorylated tau (e.g., Tau-PS262) levels between WT and the AD Tg mice. The gene discussed is MAPT; the disease is Alzheimer disease.