To determine the effect of reduced caveolin-1 expression on the long-term survival of colon cancer cells, HCT116 cells were transfected with either scrambled or caveolin-1 siRNA, treated with different concentrations of 5-FU (3.125 μg/ml, 6.25 μg/ml, 12.5 μg/ml and 25 μg/ml) and subjected to crystal violet staining 7 days after 5-FU treatment. This evidence concerns the gene CAV1 and malignant colon neoplasm.