There was no difference in overall methylation level among PTSD cases relative to controls, however the number of uniquely methylated genes did differ by disease status. Uniquely unmethylated genes in PTSD cases were associated immune system involvement, including TLR1, TLR3 (innate immune system), IL8, LTA, and KLRG-1 (adaptive immune system). This evidence concerns the gene TLR3 and post-traumatic stress disorder.