Analysis of data in the present study in Iran showed that folic acid and iron intake during pregnancy would decrease the risk for orofacial cleft which is adjusted for most studies in this field,[11][12][13] but in contrast to Cziele and Hayes reports.[14][15][16] Furthermore, inverse relationship between folic acid intake and risk for orofacial cleft is supported by findings from a large case-control study in which folic acid antagonist (dihydrofolate reductase inhibitors) was shown to increase the risk for orofacial clefts.[17]. The gene discussed is DHFR; the disease is orofacial cleft.