We firstly attempted also to fully analyze the beta globin gene in 130 blood transfusion dependent individuals in the two closely located towns, namely Eizeh and Baq-malek, After investigation a cohort of 200 beta thalassemia major patients and more than 1000 carriers in the Khuzestan Province in a previous study,[3] where we showed more than 40 mutations within different ethnic groups in the province. The gene discussed is HBB; the disease is Beta-thalassemia.