We previously demonstrated that matrix-free NCI-H226 spheroids displayed tumor diagnostic markers consistent with those of mesothelioma specimens, exhibited unique long and branching microvilli on cell surfaces, a feature characteristic of well-differentiated malignant mesothelioma in vivo, and expressed oncogenic cell junction proteins such as E-Cadherin [3]. The gene discussed is CDH1; the disease is malignant mesothelioma.