When comparing the average expression of direct Wnt targets (i.e. 24 Wnt target genes relevant to human colon and/or other cancers, with a proven direct transcriptional control through TCF binding sites as described in [29]), the SW620 cell line showed the strongest Wnt signal followed by SW480, laser-dissected tumor cells, COLO320, DLD1 and undifferentiated Caco-2 cells (Table 1, detailed description of all gene sets see supplementary data section “expression data analysis” and Additional file 1: Table S2). Here, HNF4A is linked to cancer.