Results (Figure 3E) from real time PCR reveal a marked increase in the amount of transcript for phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) genes in the high-fat diet group treated with DMSO, concordant with hyperglycemia and hyperinsulinemia. This evidence concerns the gene G6PC1 and Hyperglycemia.