Purified diabetogenic CD4+(Foxp3-depleted) TCR-HA+/− T-cells (1×104 cells/mouse) from squalene treated or untreated F1 mice were then transferred into RAG2 KO, RIP-HA Tg mice, and glycemia (read out for T1D onset) was monitored bi-weekly. The gene discussed is RAG2; the disease is type 1 diabetes mellitus.